Antibody Name: CCRC-M1
  Antibody Name: CCRC-M1
 Related Experiments: xylosyltransferases (XXTs)
Arabidopsis inflorescence stem expansion glycoprofiling
 Catolog#:
 Isotype: IgG1
 Antibody Type: Monoclonal
 Source Animal: Mouse
 Detail Info for Purified Antibody:
 Immunogen: Rhamnogalacturonan I/MeBSA complex (Non-covalent)
 Antigen: Xyloglucan, Rhamnogalacturonan I
 Plant Origin: Sycamore maple (Acer pseudoplatanus)
 Working Concentration: undiluted or diluted 1:10
 Status of Antibody: Hybridoma supernatant
 Polysaccharide Cross Reactivity: CCRC-M1.pdf
 Epitope Structure for Carbonhydrate Antigen: alpha-Fuc-(1,2)-beta-Gal
 M.W. of Protein Antigen(KDa):
 Protein Cross Reactivity:
 References: J. Puhlmann, E. Bucheli, M. J. Swain, N. Dunning, P. Albersheim, A. G. Darvill, and M. G. Hahn. (1994) Generation of monoclonal antibodies against plant cell wall polysaccharides. I. Characterization of a monoclonal antibody to a terminal alpha-(1,2)-linked fucosyl-containing epitope. Plant Physiol. 104:699-710.

G. Freshour, R. P. Clay, M. S. Fuller, P. Albersheim, A. G. Darvill, and M. G. Hahn. (1996) Developmental and tissue-specific structural alterations of the cell-wall polysaccharides of Arabidopsis thaliana roots. Plant Physiol. 110:1413-1429.

G. Freshour, C. P. Bonin, W.-D. Reiter, P. Albersheim, A. G. Darvill, and M. G. Hahn. (2003) Distribution of fucose-containing xyloglucans in cell walls of the mur1 mutant of Arabidopsis thaliana. Plant Physiol. 131:1602-1612.

Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525.

 Comments:

The epitope recognized by CCRC-M1 is most prevalent in xyloglucans from many dicots, and is present to a small extent in rhamnogalacturonan I from some dicots.

 Supplier Name: CarboSource
 Supplier Email: carbosource@ccrc.uga.edu
 Supplier Fax: (706) 542-4412
 Supplier URL: http://www.carbosource.net
 Supplier Contact Info: University of Georgia,
Complex Carbohydrate Research Center,
315 Riverbend Road, Athens,
GA ,30602-4712, USA