Antibody Name: MH4.2A4
  Antibody Name: MH4.2A4
 Related Experiments:
 Isotype: IgM
 Antibody Type: Monoclonal
 Source Animal: Mouse
 Detail Info for Purified Antibody:
 Immunogen: Leaf Protoplasts
 Antigen: Sycamore Pectic Polysaccharides
 Plant Origin: Nicotiana tabacum
 Working Concentration: undiluted or diluted 1:10
 Status of Antibody: Hybridoma supernatant
 Polysaccharide Cross Reactivity: MH4.2A4.pdf
 Epitope Structure for Carbonhydrate Antigen: unknown
 M.W. of Protein Antigen(KDa):
 Protein Cross Reactivity: arabinogalactan proteins from diverse plants
 References: Hahn MG, Lerner DR, Fitter MS, Norman PM, Lamb CJ (1987) Characterization of monoclonal antibodies to protoplast membranes of Nicotiana tabacum identified by an enzyme-linked immunosorbent assay. Planta 171:453-465.

Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525.


MH4.2A4 binds to linseed mucilage and weakly to some other arabinogalactan-containing glycans among the 54 polysaccharides tested. MH4.2A4 also binds to arabinogalactan glycoproteins.

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 Supplier Contact Info: University of Georgia,
Complex Carbohydrate Research Center,
315 Riverbend Road, Athens,
GA ,30602-4712, USA