Antibody Name: PN 16.4B4
  Antibody Name: PN 16.4B4
 Related Experiments: Arabidopsis inflorescence stem expansion glycoprofiling
 Isotype: IgG
 Antibody Type: Monoclonal
 Source Animal: Mouse
 Detail Info for Purified Antibody:
 Immunogen: Membranes from suspension-cultured cells
 Antigen: Arabinogalactan protein
 Plant Origin: Nicotiana glutinosa
 Working Concentration: Neat or 1:10 dilution of hybridoma supernatant
 Status of Antibody: Hybridoma supernatant
 Polysaccharide Cross Reactivity: PN16.4B4.pdf
 Epitope Structure for Carbonhydrate Antigen: Uncharacterized epitope in carbohydrate part of the glycoprotein
 M.W. of Protein Antigen(KDa): 135-180
 Protein Cross Reactivity: arabinogalactan proteins from diverse plants
 References: P.M. Norman, V.P.M. Wingate, M.S. Fitter, and C.J. Lamb. (1986) Monoclonal antibodies to plant plasma-membrane antigens. Planta 167:452-459.

P.M. Norman, P. Kjellbom, D.J. Bradley, M.G. Hahn, and C.J. Lamb. (1990) Immunoaffinity purification and biochemical characterization of plasma-membrane arabino-galactan-rich glycoproteins of Nicotiana glutinosa. Planta 181:365-373.

Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525.

The antibody binds to a heterogeneous mixture of arabinogalactan glycoproteins that migrate on SDS-PAGE as a broad smear ranging from 135-180 KDa. The antibody also binds strongly to gum ghatti and gum arabic.

 Supplier Name: CarboSource
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 Supplier Fax: (706) 542-4412
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 Supplier Contact Info: University of Georgia,
Complex Carbohydrate Research Center,
315 Riverbend Road, Athens,
GA ,30602-4712, USA