| Antibody Name: JIM7 | |
| Antibody Name: | JIM7 |
| Related Experiments: |
Arabidopsis inflorescence stem expansion glycoprofiling |
| Catolog#: | PP-007 |
| Isotype: | IgA |
| Antibody Type: | Monoclonal |
| Source Animal: | Rat |
| Detail Info for Purified Antibody: | |
| Immunogen: | Protoplasts prepared from suspension-cultured cells |
| Antigen: | Homogalacturonan (partially methyl-esterified) |
| Plant Origin: | Carrot (Daucus carota) |
| Working Concentration: | |
| Status of Antibody: | Hybridoma supernatant |
| Polysaccharide Cross Reactivity: | JIM7.pdf |
| Epitope Structure for Carbonhydrate Antigen: | GalA1->4MeGalA1->4MeGalA1->4MeGalA1->4MeGalA1->4GalA |
| M.W. of Protein Antigen(KDa): | |
| Protein Cross Reactivity: | |
| References: | J.P. Knox, P.J. Linstead, J. King, C. Cooper, and K. Roberts. (1990) Pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices. Planta 181:512-521. W.G.T. Willats, G. Limberg, H.C. Buchholt, G.-J. Van Alebeek, J. Benen, T.M.I.E. Christensen, J. Visser, A. Voragen, J.D. Mikkelsen, and J.P. Knox. (2000) Analysis of pectic epitopes recognised by hybridoma and phage display monoclonal antibodies using defined oligosaccharides, polysaccharides, and enzymatic degradation. Carbohydr.Res. 327:309-320, 2000. M.H. Clausen, W.G.T. Willats, and J.P. Knox. (2003) Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr.Res. 338:1797-1800, 2003. |
| Comments: | JIM7 binds to a relatively heavily methylesterified epitope found in cell walls of diverse species. The epitope structure shown is the most efficient competitor identified. Other competitors that are almost equally effective are: GalA1->4GalA1->4MeGalA1->4MeGalA1->4MeGalA1->4GalA and MeGalA1->4GalA1->4MeGalA1->4GalA1->4MeGalA1->4GalA. |
| Supplier Name: | CarboSource |
| Supplier Email: | carbosource@ccrc.uga.edu |
| Supplier Fax: | (706) 542-4412 |
| Supplier URL: | http://www.carbosource.net |
| Supplier Contact Info: | University of Georgia, Complex Carbohydrate Research Center, 315 Riverbend Road, Athens, GA ,30602-4712, USA |
