Antibody Name: JIM5
  Antibody Name: JIM5
 Related Experiments: Arabidopsis inflorescence stem expansion glycoprofiling
 Isotype: IgG2a
 Antibody Type: Monoclonal
 Source Animal: Rat
 Detail Info for Purified Antibody:
 Immunogen: Protoplast prepared from suspension-cultured cells
 Antigen: Homogalacturonan (partially methyl-esterified)
 Plant Origin: Carrot (Daucus carota)
 Working Concentration:
 Status of Antibody: Hybridoma supernatant
 Polysaccharide Cross Reactivity: JIM5.pdf
 Epitope Structure for Carbonhydrate Antigen: Me(alpha)GalA1->4(alpha)GalA1->4(alpha)GalA1->4(alpha)GalA1->4(alpha)GalA1->4(alpha)MeGalA
 M.W. of Protein Antigen(KDa):
 Protein Cross Reactivity:
 References: K.A. VandenBosch, D.J. Bradley, J.P. Knox, S. Perotto, G.W. Butcher, and N.J. Brewin. (1989) Common components of the infection thread matrix and the intercellular space identified by immunocytochemical analysis of pea nodules and uninfected roots. EMBO J. 8:335-342.

J.P. Knox, P.J. Linstead, J. King, C. Cooper, and K. Roberts. (1990) Pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices. Planta 181:512-521.

W.G.T. Willats, G. Limberg, H.C. Buchholt, G.-J. Van Alebeek, J. Benen, T. M.I.E. Christensen, J. Visser, A. Voragen, J.D. Mikkelsen, and J.P. Knox. (2000) Analysis of pectic epitopes recognised by hybridoma and phage display monoclonal antibodies using defined oligosaccharides, polysaccharides, and enzymatic degradation. Carbohydr.Res. 327:309-320.

M.H. Clausen, W.G.T. Willats, and J.P. Knox. (2003) Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr.Res. 338:1797-1800.


JIM5 binds to a sparsely methylated homogalacturonan epitope found in the walls of diverse plant species. The epitope structure listed above is the most effective structure identified to date. JIM5 also binds to unesterified homogalacturonan.

JIM5 can also be obtained from Plant Probes (

 Supplier Name: CarboSource
 Supplier Email:
 Supplier Fax: (706) 542-4412
 Supplier URL:
 Supplier Contact Info: University of Georgia,
Complex Carbohydrate Research Center,
315 Riverbend Road, Athens,
GA ,30602-4712, USA