Antibody Name: LM15
  Antibody Name: LM15
 Related Experiments:
 Catolog#: ELD013
 Isotype: IgG2c
 Antibody Type: Monoclonal
 Source Animal: Rat
 Detail Info for Purified Antibody:
 Immunogen: XXXG-BSA
 Antigen: Xyloglucan
 Plant Origin:
 Working Concentration: 1:10 dilution
 Status of Antibody: Hybridoma supernatant
 Polysaccharide Cross Reactivity: LM15.pdf
 Epitope Structure for Carbonhydrate Antigen: GX-
 M.W. of Protein Antigen(KDa):
 Protein Cross Reactivity:
 References: S.E. Marcus, Y. Verhertbruggen, C. Herve, J. J. Ordaz-Ortiz, V. Farkas, H.L. Pedersen, W.G.T. Willats, and J.P. Knox. 2008. Pectic homogalacturonan masks abundant sets of xyloglucan epitopes in plant cell walls. BMC Plant Biology 8:60-71.

Ruprecht C, Bartetzko MP, Senf D, Dallabernardina P, Boos I, Andersen MCF, Kotake T, Knox JP, Hahn MG, Clausen MH, Pfrengle F (2017) A synthetic glycan microarray enables epitope mapping of plant cell wall glycan-directed antibodies. Plant Physiology 175:1094-1104.

K. Thorne, B.R. Urbanowicz, M.G. Hahn (2024) Plant cell wall glycan-directed monoclonal antibodies. In A. Geitmann, ed, Plant Cell Walls: Research Milestones and Conceptual Insights. CRC Press, Boca Raton, FL, USA, pp 206-236.

 Comments:

LM15 binds to the non-reducing terminal GX of xyloglucan chains. The structure of the other two side-chains in the xyloglucan oligosaccharide repeat structure do not affect antibody binding.

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