Antibody Name: 2F4
  Antibody Name: 2F4
 Related Experiments:
 Catolog#: PP-2F4
 Isotype: IgG1
 Antibody Type: Monoclonal
 Source Animal: Mouse
 Detail Info for Purified Antibody:
 Immunogen: Polygalacturonic acid
 Antigen: Homogalacturonan
 Plant Origin:
 Working Concentration: up to 1:250 of hybridoma supernatant
 Status of Antibody: Hybridoma supernatant
 Polysaccharide Cross Reactivity:
 Epitope Structure for Carbonhydrate Antigen: dimeric association of pectic chains through calcium ions
 M.W. of Protein Antigen(KDa):
 Protein Cross Reactivity:
 References: Liners, F., Letesson, J.-J., Didembourg, C., Van Cutsem, P. (1989) Monoclonal antibodies against pectin. Recognition of a conformation induced by calcium. Plant Physiol. 91:1419-1424

Liners, F., Thibault, J.-F., Van Cutsem, P. (1992) Influence of the degree of polymerization of oligogalacturonates and of esterification pattern of pecin on their recognition by monoclonal antibodies. Plant Physiol. 99:1099-1104

Liners, F., Van Cutsem, P. (1992) Distribution of pectic polysaccharide throughout walls of suspension-cultured carrot cells. An immunocytochemical study. Protoplasma 170:10-21.

 Comments:

This antibody binds to homogalacturonans of low degrees of methylesterification (DM) up to 40%.

This antibody DOES NOT work in conventional PBS or TBS buffers. For optimal results, use the following T/Ca/S buffer: Tris-HCl 20 mM pH 8.2, CaCl2 0.5 mM, NaCl 150 mM.

Questions regarding this antibody can be directed to Dr. Francoise Liners at francoise.liners@fundp.ac.be.

 Supplier Name: PlantProbes
 Supplier Email: antibodies@plantprobes.co.uk
 Supplier Fax: 44-(0)113-3433144
 Supplier URL: http://www.plantprobes.net
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