| Antibody Name: 2F4 | |
| Antibody Name: | 2F4 |
| Related Experiments: |
Fadera |
| Catolog#: | PP-2F4 |
| Isotype: | IgG1 |
| Antibody Type: | Monoclonal |
| Source Animal: | Mouse |
| Detail Info for Purified Antibody: | |
| Immunogen: | Polygalacturonic acid |
| Antigen: | Homogalacturonan |
| Plant Origin: | |
| Working Concentration: | up to 1:250 of hybridoma supernatant |
| Status of Antibody: | Hybridoma supernatant |
| Polysaccharide Cross Reactivity: | |
| Epitope Structure for Carbonhydrate Antigen: | dimeric association of pectic chains through calcium ions |
| M.W. of Protein Antigen(KDa): | |
| Protein Cross Reactivity: | |
| References: | Liners, F., Letesson, J.-J., Didembourg, C., Van Cutsem, P. (1989) Monoclonal antibodies against pectin. Recognition of a conformation induced by calcium. Plant Physiol. 91:1419-1424 Liners, F., Thibault, J.-F., Van Cutsem, P. (1992) Influence of the degree of polymerization of oligogalacturonates and of esterification pattern of pecin on their recognition by monoclonal antibodies. Plant Physiol. 99:1099-1104 Liners, F., Van Cutsem, P. (1992) Distribution of pectic polysaccharide throughout walls of suspension-cultured carrot cells. An immunocytochemical study. Protoplasma 170:10-21. |
| Comments: | This antibody binds to homogalacturonans of low degrees of methylesterification (DM) up to 40%. This antibody DOES NOT work in conventional PBS or TBS buffers. For optimal results, use the following T/Ca/S buffer: Tris-HCl 20 mM pH 8.2, CaCl2 0.5 mM, NaCl 150 mM. Questions regarding this antibody can be directed to Dr. Francoise Liners at francoise.liners@fundp.ac.be. |
| Supplier Name: | PlantProbes |
| Supplier Email: | antibodies@plantprobes.co.uk |
| Supplier Fax: | 44-(0)113-3433144 |
| Supplier URL: | http://www.plantprobes.net |
| Supplier Contact Info: | , , , Leeds, ,, United Kingdon |
