| Antibody Name: CCRC-M154 | |
| Antibody Name: | CCRC-M154 |
| Related Experiments: | |
| Catolog#: | |
| Isotype: | IgM |
| Antibody Type: | Monoclonal |
| Source Animal: | Mouse |
| Detail Info for Purified Antibody: | |
| Immunogen: | xylan/BSA covalent conjugate |
| Antigen: | Xylan (Corn stover BH2) |
| Plant Origin: | Zea mays |
| Working Concentration: | undiluted or diluted 1:10 |
| Status of Antibody: | Hybridoma supernatant |
| Polysaccharide Cross Reactivity: | CCRC-M154.pdf |
| Epitope Structure for Carbonhydrate Antigen: | alpha-Ara(1,3)-linked to beta-(1,4)-linked xylan backbone |
| M.W. of Protein Antigen(KDa): | |
| Protein Cross Reactivity: | |
| References: | Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525. Schmidt D, Schuhmacher F, Geissner A, Seeberger PH, Pfrengle F (2015) Automated synthesis of arabinoxylan-oligosaccharides enables characterization of antibodies that recognize plant cell wall glycans. Chemistry-A European Journal 21:5709-5713. Ruprecht C, Bartetzko MP, Senf D, Dallabernardina P, Boos I, Andersen MCF, Kotake T, Knox JP, Hahn MG, Clausen MH, Pfrengle F (2017) A synthetic glycan microarray enables epitope mapping of plant cell wall glycan-directed antibodies. Plant Physiology 175:1094-1104. K. Thorne, B.R. Urbanowicz, M.G. Hahn (2024) Plant cell wall glycan-directed monoclonal antibodies. In A. Geitmann, ed, Plant Cell Walls: Research Milestones and Conceptual Insights. CRC Press, Boca Raton, FL, USA, pp 206-236, https://www.researchgate.net/publication/376031352_Plant_Cell_Wall_Glycan-Directed_Monoclonal_Antibodies |
| Comments: | This antibody only binds to arabinosyl side-chains of xylans. It does not bind to the naked xylan backbone or any other side-chain substituents on xylans. The antibody also does not bind to other classes of plant cell wall glycans. |
| Supplier Name: | CarboSource |
| Supplier Email: | carbosource@ccrc.uga.edu |
| Supplier Fax: | (706) 542-4412 |
| Supplier URL: | http://www.carbosource.net |
| Supplier Contact Info: | University of Georgia, Complex Carbohydrate Research Center, 315 Riverbend Road, Athens, GA ,30602-4712, USA |
