| Antibody Name: CCRC-M155 | |
| Antibody Name: | CCRC-M155 |
| Related Experiments: | |
| Catolog#: | |
| Isotype: | IgG1 |
| Antibody Type: | Monoclonal |
| Source Animal: | Mouse |
| Detail Info for Purified Antibody: | |
| Immunogen: | Xylan/cBSA covalent conjugate |
| Antigen: | Corn stover xylan |
| Plant Origin: | Zea maize |
| Working Concentration: | undiluted or diluted 1:10 |
| Status of Antibody: | Hybridoma supernatant |
| Polysaccharide Cross Reactivity: | CCRC-M155.pdf |
| Epitope Structure for Carbonhydrate Antigen: | 4-O-Me-alpha-GlcA-(1,2)-linked to beta-(1,4)-linked xylan backbone |
| M.W. of Protein Antigen(KDa): | |
| Protein Cross Reactivity: | |
| References: | Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525. Ruprecht C, Bartetzko MP, Senf D, Dallabernardina P, Boos I, Andersen MCF, Kotake T, Knox JP, Hahn MG, Clausen MH, Pfrengle F (2017) A synthetic glycan microarray enables epitope mapping of plant cell wall glycan-directed antibodies. Plant Physiology 175:1094-1104. K. Thorne, B.R. Urbanowicz, M.G. Hahn (2024) Plant cell wall glycan-directed monoclonal antibodies. In A. Geitmann, ed, Plant Cell Walls: Research Milestones and Conceptual Insights. CRC Press, Boca Raton, FL, USA, pp 206-236. |
| Comments: | This antibody recognizes the 4-O-methyl-GlcA side-chains of xylans. The presence of the methyl group is required for antibody binding. The antibody does not recognize the unsubstituted beta-(1,4)-linked xylan backbone. This antibody binds only to xylans; it does not bind to any other classes of plant cell wall glycans that have been tested. |
| Supplier Name: | CarboSource |
| Supplier Email: | carbosource@ccrc.uga.edu |
| Supplier Fax: | (706) 542-4412 |
| Supplier URL: | http://www.carbosource.net |
| Supplier Contact Info: | University of Georgia, Complex Carbohydrate Research Center, 315 Riverbend Road, Athens, GA ,30602-4712, USA |
