Antibody Name: MAC207 | |
Antibody Name: | MAC207 |
Related Experiments: |
Arabidopsis inflorescence stem expansion glycoprofiling |
Catolog#: | |
Isotype: | IgM |
Antibody Type: | Monoclonal |
Source Animal: | Rat |
Detail Info for Purified Antibody: | |
Immunogen: | Peribacteroid Membrane |
Antigen: | Arabinogalactan Protein |
Plant Origin: | Pea (Pisum sativum) |
Working Concentration: | Undiluted |
Status of Antibody: | Hybridoma supernatant |
Polysaccharide Cross Reactivity: | MAC207.pdf |
Epitope Structure for Carbonhydrate Antigen: | (beta)GlcA1->3(alpha)GalA1->2Rha |
M.W. of Protein Antigen(KDa): | 70-100 |
Protein Cross Reactivity: | Arabinogalactan proteins from diverse plants |
References: | D.J. Bradley, E.A. Wood, A.P. Larkins, G. Galfre, G.W. Butcher, and N.J. Brewin. (1988) Isolation of monoclonal antibodies reacting with peribacteroid membranes and other components of pea root nodules containing Rhizobium leguminosarum. Planta 173:149-160. R.I. Pennell, J.P. Knox, G.N. Scofield, R. Selvendran, and K. Roberts. (1989) A family of abundant plasma membrane-associated glycoproteins related to the arabinogalactan proteins is unique to flowering plants. J.Cell Biol. 108:1967-1977. E.A. Yates and J.P. Knox. (1994) Investigations into the occurrence of plant cell surface epitopes in exudate gums. Carbohydr.Polym. 24:281-286. E.A. Yates, J.-F. Valdor, S.M. Haslam, H.R. Morris, A. Dell, W. Mackie, and J.P. Knox. (1996) Characterization of carbohydrate structural features recognized by anti-arabinogalactan-protein monoclonal antibodies. Glycobiology 6:131-139. Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525. |
Comments: | MAC207 binds to various exudate gums, including gum arabic, gum ghatti, gum karaya, and gum tragacanth. The oligosaccharide shown above is the most effective oligosaccharide competitor tested to date. |
Supplier Name: | CarboSource |
Supplier Email: | carbosource@ccrc.uga.edu |
Supplier Fax: | (706) 542-4412 |
Supplier URL: | http://www.carbosource.net |
Supplier Contact Info: | University of Georgia, Complex Carbohydrate Research Center, 315 Riverbend Road, Athens, GA ,30602-4712, USA |