| Antibody Name: CCRC-M169 | |
| Antibody Name: | CCRC-M169 |
| Related Experiments: | |
| Catolog#: | |
| Isotype: | IgM |
| Antibody Type: | Monoclonal |
| Source Animal: | Mouse |
| Detail Info for Purified Antibody: | |
| Immunogen: | Mannan/cBSA covalent conjugate |
| Antigen: | Acetylated mannan |
| Plant Origin: | Aloe vera |
| Working Concentration: | undiluted or diluted 1:10 |
| Status of Antibody: | Hybridoma supernatant |
| Polysaccharide Cross Reactivity: | CCRC-M169.pdf |
| Epitope Structure for Carbonhydrate Antigen: | beta-(1,4)-Man-beta-(1,4)-OAc-Man-beta-(1,4)-Man- |
| M.W. of Protein Antigen(KDa): | |
| Protein Cross Reactivity: | |
| References: | Zhang X, Rogowski A, Zhao L, Hahn MG, Avci U, Knox JP, Gilbert HJ (2014) Understanding how the complex molecular architecture of mannan-degrading hydrolases contributes to plant cell wall degradation. Journal of Biological Chemistry 289:2002-2012. K. Thorne, B.R. Urbanowicz, M.G. Hahn (2024) Plant cell wall glycan-directed monoclonal antibodies. In A. Geitmann, ed, Plant Cell Walls: Research Milestones and Conceptual Insights. CRC Press, Boca Raton, FL, USA, pp 206-236. |
| Comments: | CCRC-M169 binds to acetylated beta-(1,4)-linked mannans. It is unknown if the antibody is selective for O-2 or O-3 acetylation; both are present in acetylated mannans. Removal of the acetyl groups abolishes antibody binding. CCRC-M169 binds only to tomato glucomannan among the 54 plant polysaccharides tested. |
| Supplier Name: | CarboSource |
| Supplier Email: | carbosource@ccrc.uga.edu |
| Supplier Fax: | (706) 542-4412 |
| Supplier URL: | http://www.carbosource.net |
| Supplier Contact Info: | University of Georgia, Complex Carbohydrate Research Center, 315 Riverbend Road, Athens, GA ,30602-4712, USA |
