Antibody Name: MAC204
  Antibody Name: MAC204
 Related Experiments: Arabidopsis inflorescence stem expansion glycoprofiling
 Isotype: IgM
 Antibody Type: Monoclonal
 Source Animal: Rat
 Detail Info for Purified Antibody:
 Immunogen: Peribacteroid membrane
 Antigen: Arabinogalactan protein
 Plant Origin: Pea (Pisum sativum)
 Working Concentration:
 Status of Antibody: Hybridoma supernatant
 Polysaccharide Cross Reactivity: MAC204.pdf
 Epitope Structure for Carbonhydrate Antigen: arabinogalactan (epitope structure unknown)
 M.W. of Protein Antigen(KDa): 90-100
 Protein Cross Reactivity:
 References: D.J. Bradley, E.A. Wood, A.P. Larkins, G. Galfre, G.W. Butcher, and N.J. Brewin. (1988) Isolation of monoclonal antibodies reacting with peribacteroid membranes and other components of pea root nodules containing Rhizobium leguminosarum. Planta 173:149-160.

K.A. VandenBosch, D.J. Bradley, J.P. Knox, S. Perotto, G.W. Butcher, and N.J. Brewin. (1989) Common components of the infection thread matrix and the intercellular space identified by immunocytochemical analysis of pea nodules and uninfected roots. EMBO J. 8:335-342.

Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525.


MAC204 binds to arabinogalactan proteins in both Rhizobium-infected and non-infected tissues. MAC204 also cross-reacts with the exudate gum, gum arabic.

The epitope recognized by MAC204 has not been characterized in detail, except that it appears to be carbohydrate in nature (i.e., periodate sensitive).

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 Supplier Contact Info: University of Georgia,
Complex Carbohydrate Research Center,
315 Riverbend Road, Athens,
GA ,30602-4712, USA