Antibody Name: CCRC-M7 | |
Antibody Name: | CCRC-M7 |
Related Experiments: |
Arabidopsis inflorescence stem expansion glycoprofiling |
Catolog#: | |
Isotype: | IgG1 |
Antibody Type: | Monoclonal |
Source Animal: | Mouse |
Detail Info for Purified Antibody: | |
Immunogen: | Rhamnogalacturonan I/MeBSA complex (Non-covalent) |
Antigen: | Rhamnogalacturonan I |
Plant Origin: | Sycamore maple (Acer pseudoplatanus) |
Working Concentration: | Neat or diluted 1:10 |
Status of Antibody: | Hybridoma supernatant |
Polysaccharide Cross Reactivity: | CCRC-M7.pdf |
Epitope Structure for Carbonhydrate Antigen: | trimer or larger of beta-(1,6)-Gal carrying one or more Ara residues of unknown linkage |
M.W. of Protein Antigen(KDa): | |
Protein Cross Reactivity: | |
References: | J. Puhlmann, E. Bucheli, M. J. Swain, N. Dunning, P. Albersheim, A. G. Darvill, and M. G. Hahn. Generation of monoclonal antibodies against plant cell wall polysaccharides. I. Characterization of a monoclonal antibody to a terminal alpha-1,2-linked fucosyl-containing epitope. Plant Physiol. 104:699-710, 1994. W. Steffan, P. Kovac, P. Albersheim, A. G. Darvill, and M. G. Hahn. Characterization of a monoclonal antibody that recognizes an arabinosylated (1,6)-beta-D-galactan epitope in plant complex carbohydrates. Carbohydr.Res. 275:295-307, 1995. Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525. |
Comments: | The epitope recognized by CCRC-M7 is present on rhamnogalacturonan I, and on soluble and membrane-bound arabinogalactan glycoproteins |
Supplier Name: | CarboSource |
Supplier Email: | carbosource@ccrc.uga.edu |
Supplier Fax: | (706) 542-4412 |
Supplier URL: | http://www.carbosource.net |
Supplier Contact Info: | University of Georgia, Complex Carbohydrate Research Center, 315 Riverbend Road, Athens, GA ,30602-4712, USA |