Antibody Name: JIM13
  Antibody Name: JIM13
 Related Experiments: Arabidopsis inflorescence stem expansion glycoprofiling
 Isotype: IgM
 Antibody Type: Monoclonal
 Source Animal: Rat
 Detail Info for Purified Antibody:
 Immunogen: Arabinogalactan protein (AGP2)
 Antigen: Arabinogalactan, Arabinogalactan protein
 Plant Origin: Carrot (Daucus carota)
 Working Concentration: Neat or diluted 1:10
 Status of Antibody: Hybridoma supernatant
 Polysaccharide Cross Reactivity: JIM13.pdf
 Epitope Structure for Carbonhydrate Antigen: (beta)GlcA1->3(alpha)GalA1->2Rha
 M.W. of Protein Antigen(KDa): 80-100
 Protein Cross Reactivity: Arabinogalactan proteins from diverse plants
 References: J. P. Knox, P. J. Linstead, J. Peart, C. Cooper, and K. Roberts. (1991) Developmentally-regulated epitopes of cell surface arabinogalactan-proteins and their relation to root tissue pattern formation. Plant Journal 1:317-326.

E. A. Yates and J. P. Knox. (1994) Investigations into the occurrence of plant cell surface epitopes in exudate gums. Carbohydr.Polym. 24:281-286.

E. A. Yates, J.-F. Valdor, S. M. Haslam, H. R. Morris, A. Dell, W. Mackie, and J. P. Knox. (1996) Characterization of carbohydrate structural features recognized by anti-arabinogalactan-protein monoclonal antibodies. Glycobiology 6:131-139.


JIM13 binds to various exudate gums, including gum arabic and gum ghatti. JIM13 also binds to rhamnogalacturonan I from sycamore maple. The oligosaccharide shown above is the most effective oligosaccharide competitor tested to date.

 Supplier Name: CarboSource
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 Supplier Contact Info: University of Georgia,
Complex Carbohydrate Research Center,
315 Riverbend Road, Athens,
GA ,30602-4712, USA