Antibody Name: JIM13 | |
Antibody Name: | JIM13 |
Related Experiments: |
Arabidopsis inflorescence stem expansion glycoprofiling |
Catolog#: | |
Isotype: | IgM |
Antibody Type: | Monoclonal |
Source Animal: | Rat |
Detail Info for Purified Antibody: | |
Immunogen: | Arabinogalactan protein (AGP2) |
Antigen: | Arabinogalactan, Arabinogalactan protein |
Plant Origin: | Carrot (Daucus carota) |
Working Concentration: | Neat or diluted 1:10 |
Status of Antibody: | Hybridoma supernatant |
Polysaccharide Cross Reactivity: | JIM13.pdf |
Epitope Structure for Carbonhydrate Antigen: | (beta)GlcA1->3(alpha)GalA1->2Rha |
M.W. of Protein Antigen(KDa): | 80-100 |
Protein Cross Reactivity: | Arabinogalactan proteins from diverse plants |
References: | J. P. Knox, P. J. Linstead, J. Peart, C. Cooper, and K. Roberts. (1991) Developmentally-regulated epitopes of cell surface arabinogalactan-proteins and their relation to root tissue pattern formation. Plant Journal 1:317-326. E. A. Yates and J. P. Knox. (1994) Investigations into the occurrence of plant cell surface epitopes in exudate gums. Carbohydr.Polym. 24:281-286. E. A. Yates, J.-F. Valdor, S. M. Haslam, H. R. Morris, A. Dell, W. Mackie, and J. P. Knox. (1996) Characterization of carbohydrate structural features recognized by anti-arabinogalactan-protein monoclonal antibodies. Glycobiology 6:131-139. |
Comments: | JIM13 binds to various exudate gums, including gum arabic and gum ghatti. JIM13 also binds to rhamnogalacturonan I from sycamore maple. The oligosaccharide shown above is the most effective oligosaccharide competitor tested to date. |
Supplier Name: | CarboSource |
Supplier Email: | carbosource@ccrc.uga.edu |
Supplier Fax: | (706) 542-4412 |
Supplier URL: | http://www.carbosource.net |
Supplier Contact Info: | University of Georgia, Complex Carbohydrate Research Center, 315 Riverbend Road, Athens, GA ,30602-4712, USA |