Antibody Name: JIM14
  Antibody Name: JIM14
 Related Experiments: Arabidopsis inflorescence stem expansion glycoprofiling
 Isotype: IgM
 Antibody Type: Monoclonal
 Source Animal: Rat
 Detail Info for Purified Antibody:
 Immunogen: Arabinogalactan protein (AGP2)
 Antigen: Arabinogalactan, Arabinogalactan protein
 Plant Origin: Carrot (Daucus carota)
 Working Concentration: Neat or diluted 1:10
 Status of Antibody: Hybridoma supernatant
 Polysaccharide Cross Reactivity: JIM14.pdf
 Epitope Structure for Carbonhydrate Antigen: unknown
 M.W. of Protein Antigen(KDa): 80-100
 Protein Cross Reactivity: Arabinogalactan proteins from diverse plants
 References: J. P. Knox, P. J. Linstead, J. Peart, C. Cooper, and K. Roberts. (1991) Developmentally-regulated epitopes of cell surface arabinogalactan-proteins and their relation to root tissue pattern formation. Plant Journal 1:317-326.

E. A. Yates and J. P. Knox. (1994) Investigations into the occurrence of plant cell surface epitopes in exudate gums. Carbohydr.Polym. 24:281-286.

E. A. Yates, J.-F. Valdor, S. M. Haslam, H. R. Morris, A. Dell, W. Mackie, and J. P. Knox. (1996) Characterization of carbohydrate structural features recognized by anti-arabinogalactan-protein monoclonal antibodies. Glycobiology 6:131-139.


JIM14 binds to rhamnogalacturonan I from sycamore maple and to the exudate gum, gum ghatti. The epitope structure recognized by JIM14 is unknown. Immunolocalization data suggest that JIM14 recognizes an epitope distinct from JIM13 and JIM15.

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 Supplier Contact Info: University of Georgia,
Complex Carbohydrate Research Center,
315 Riverbend Road, Athens,
GA ,30602-4712, USA