Antibody Name: CCRC-M34
  Antibody Name: CCRC-M34
 Related Experiments: Arabidopsis inflorescence stem expansion glycoprofiling
 Isotype: IgM
 Antibody Type: Monoclonal
 Source Animal: Mouse
 Detail Info for Purified Antibody:
 Immunogen: Seed mucillage/MeBSA complex (Non-covalent)
 Antigen: Homogalacturonan (partially methyl-esterified)
 Plant Origin: Arabidopsis thaliana
 Working Concentration: undiluted
 Status of Antibody: Hybridoma supernatant
 Polysaccharide Cross Reactivity: CCRC-M34.pdf
 Epitope Structure for Carbonhydrate Antigen: Base-sensitive homogalacturonan epitope
 M.W. of Protein Antigen(KDa):
 Protein Cross Reactivity:
 References: Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525.

CCRC-M34 binds to a base-labile epitope in homogalacturonan, presumably recognizing a pattern of methyl-esterification. Hierarchical clustering of polysaccharide ELISA data groups CCRC-M34 with JIM7, but immunolocalization data suggest that CCRC-34 binds to an epitope distinct from that recognized by JIM7.

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 Supplier Contact Info: University of Georgia,
Complex Carbohydrate Research Center,
315 Riverbend Road, Athens,
GA ,30602-4712, USA