Antibody Name: JIM4
  Antibody Name: JIM4
 Related Experiments: Arabidopsis inflorescence stem expansion glycoprofiling
 Isotype: IgM
 Antibody Type: Monoclonal
 Source Animal: Rat
 Detail Info for Purified Antibody:
 Immunogen: Protoplasts from suspension cultured cells
 Antigen: Arabinogalactan glycoprotein
 Plant Origin: Carrot (Daucus carota)
 Working Concentration: Neat
 Status of Antibody: Hybridoma supernatant
 Polysaccharide Cross Reactivity: JIM4.pdf
 Epitope Structure for Carbonhydrate Antigen: betaGlcA-(1,3)-alphaGalA-(1,2)-Rha
 M.W. of Protein Antigen(KDa): 70-100
 Protein Cross Reactivity:
 References: J. P. Knox, S. Day, and K. Roberts. (1989) A set of cell surface glycoproteins forms an early marker of cell position, but not cell type, in the root apical meristem of Daucus carota L. Development 106:47-56.

E. A. Yates and J. P. Knox. (1994) Investigations into the occurrence of plant cell surface epitopes in exudate gums. Carbohydr.Polym. 24:281-286.

E. A. Yates, J.-F. Valdor, S. M. Haslam, H. R. Morris, A. Dell, W. Mackie, and J. P. Knox. (1996) Characterization of carbohydrate structural features recognized by anti-arabinogalactan-protein monoclonal antibodies. Glycobiology 6:131-139.


JIM4 binds to a set of discreet protein bands MW 20-60 KDa in addition to the smear indicated above. Binding is not competable with monosaccharides. The most competitive oligosaccharide tested to date is the one indicated above. JIM4 also binds to several exudate gums, including gum arabic and gum ghatti.

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