| Antibody Name: JIM4 | |
| Antibody Name: | JIM4 |
| Related Experiments: |
Arabidopsis inflorescence stem expansion glycoprofiling |
| Catolog#: | |
| Isotype: | IgM |
| Antibody Type: | Monoclonal |
| Source Animal: | Rat |
| Detail Info for Purified Antibody: | |
| Immunogen: | Protoplasts from suspension cultured cells |
| Antigen: | Arabinogalactan glycoprotein |
| Plant Origin: | Carrot (Daucus carota) |
| Working Concentration: | Neat |
| Status of Antibody: | Hybridoma supernatant |
| Polysaccharide Cross Reactivity: | JIM4.pdf |
| Epitope Structure for Carbonhydrate Antigen: | betaGlcA-(1,3)-alphaGalA-(1,2)-Rha |
| M.W. of Protein Antigen(KDa): | 70-100 |
| Protein Cross Reactivity: | |
| References: | J. P. Knox, S. Day, and K. Roberts. (1989) A set of cell surface glycoproteins forms an early marker of cell position, but not cell type, in the root apical meristem of Daucus carota L. Development 106:47-56. E. A. Yates and J. P. Knox. (1994) Investigations into the occurrence of plant cell surface epitopes in exudate gums. Carbohydr.Polym. 24:281-286. E. A. Yates, J.-F. Valdor, S. M. Haslam, H. R. Morris, A. Dell, W. Mackie, and J. P. Knox. (1996) Characterization of carbohydrate structural features recognized by anti-arabinogalactan-protein monoclonal antibodies. Glycobiology 6:131-139. |
| Comments: | JIM4 binds to a set of discreet protein bands MW 20-60 KDa in addition to the smear indicated above. Binding is not competable with monosaccharides. The most competitive oligosaccharide tested to date is the one indicated above. JIM4 also binds to several exudate gums, including gum arabic and gum ghatti. |
| Supplier Name: | CarboSource |
| Supplier Email: | carbosource@ccrc.uga.edu |
| Supplier Fax: | (706) 542-4412 |
| Supplier URL: | http://www.carbosource.net |
| Supplier Contact Info: | University of Georgia, Complex Carbohydrate Research Center, 315 Riverbend Road, Athens, GA ,30602-4712, USA |
