Antibody Name: MH4.4E4
  Antibody Name: MH4.4E4
 Related Experiments:
 Isotype: IgM
 Antibody Type: Monoclonal
 Source Animal: Mouse
 Detail Info for Purified Antibody:
 Immunogen: Leaf Protoplasts
 Antigen: Rhamnogalacturonan I
 Plant Origin: Nicotiana tabacum
 Working Concentration: undiluted or diluted 1:10
 Status of Antibody: Hybridoma supernatant
 Polysaccharide Cross Reactivity: MH4.4E4.pdf
 Epitope Structure for Carbonhydrate Antigen: unknown
 M.W. of Protein Antigen(KDa):
 Protein Cross Reactivity: arabinogalactan proteins from diverse plants
 References: Hahn MG, Lerner DR, Fitter MS, Norman PM, Lamb CJ (1987) Characterization of monoclonal antibodies to protoplast membranes of Nicotiana tabacum identified by an enzyme-linked immunosorbent assay. Planta 171:453-465.

Pattathil S, Avci U, Baldwin D, Swennes AG, McGill JA, Popper Z, Bootten T, Albert A, Davis RH, Chennareddy C, Dong R, O'Shea B, Rossi R, Leoff C, Freshour G, Narra R, O'Neil M, York WS, Hahn MG. (2010) A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies. Plant Physiol. 153:514-525.


MH4.4E4 binds only to linseed mucilage among the 54 polysaccharides tested. Mh4.4E4 also binds to arabinogalactan glycoproteins.

 Supplier Name: CarboSource
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 Supplier Contact Info: University of Georgia,
Complex Carbohydrate Research Center,
315 Riverbend Road, Athens,
GA ,30602-4712, USA