Mutant Name: cals5
| Gene Name | CalS5 |
| Protein Name | Callose synthase |
| Mutagen | T-DNA |
| Category | reverse genetics |
| Organism | Arabidopsis thaliana |
| Ecotype | Col |
| Donor | |
| General Description |
1.There were no observable differences in the vegetative parts of the cals5 mutant and wild-type plants. 2. the mutant plants developed abnormal flowers with shrunken anthers and were partially or completely sterile. |
| Organ Specificity | The CalS5 gene is also expressed in other organs with the highest expression in meiocytes, tetrads, microspores and mature pollen. |
| Impact on Wall | the pollen exine wall was not formed properly, affecting the baculae and tectum structure and tryphine was deposited randomly as globular structures. |
| Impact on Sugar Composition | Callose deposition in the cals5 mutant was nearly completely lacking. |
| Reference | Dong X, Hong Z, Sivaramakrishnan M, Mahfouz M, Verma DP. Callose synthase (CalS5) is required for exine formation during microgametogenesis and for pollen viability in Arabidopsis. Plant J. 2005 May;42(3):315-28. |