Mutant Name: csla7
| Gene Name | CslA7 |
| Protein Name | mannan synthase |
| Mutagen | Ds transposon |
| Category | reverse genetics |
| Organism | Arabidopsis |
| Ecotype | Lb |
| Donor | |
| General Description |
1. The mutant gene has a transposon insretion at the 7th exon. 2. The homozygous insertion was embryo lethal. 3. Mutant pollen developed normally, but its transmission efficiency was reduced by 71% compared with the WT. |
| Organ Specificity |
1. The gene is ubiquitously expressed. 2. The mutant embryos showed abnormal cell patterning and they arrested at a globular stage. 3. AtCSLA7 is important for pollen tube growth. |
| Impact on Wall |
1. The defective embryonic development was associated with reduced proliferation and failed cellularization of the endosperm. 2. The defect in pollen tube growth could result from changes in cell wall properties,including its extensibility and/or stability as a result of the absence of a specific polysaccaride. |
| Impact on Sugar Composition | not checked |
| Reference |
1.Goubet F, Misrahi A, Park SK, Zhang Z, Twell D, Dupree P. (2003) AtCSLA7, a cellulose synthase-like putative glycosyltransferase, is important for pollen tube growth and embryogenesis in Arabidopsis. Plant Physiol. Feb;131(2):547-57. 2.Liepman AH, Wilkerson CG, Keegstra K. Expression of cellulose synthase-like (Csl) genes in insect cells reveals that CslA family members encode mannan synthases. Proc Natl Acad Sci U S A. 2005 Feb 8;102(6):2221-6. |