Mutant Name: dgl1
| Gene Name | DGL1 |
| Protein Name | oligosaccharyltransferase (putative) |
| Mutagen | T-DNA |
| Category | forward genetics |
| Organism | Arabidopsis thaliana |
| Ecotype | Ws |
| Donor | |
| General Description |
1.The dgl1-1 mutant(defective glycosylation1-1) was dwarf; it was identified in Arabidopsis based on a growth defect of the dark-grown hypocotyl and an abnormal composition of the non-cellulosic cell wall polysaccharides,. 2.The mutation was caused by a T-DNA insertion in chromosome5,62bp upstream of the ATG of the gene At5g66680, annotated as encoding a putative subunit of the oligosaccharyltransferase(OST) complex. the dgl1-1 mutation led to a reduced N-linked glycosylation of the ER-resident protein disulfide isomerase. 3.DGL1 is highly similar to yeast WBP1 and human OST48, which are subunits of the OST complex. |
| Organ Specificity | |
| Impact on Wall | Microscopic analysis of dgl1-1 revealed developmental defects including reduced cell elongation and the collapse and differentiation defects of cells in the central cylinder. These defects were accompanied by changes in the non-cellulosic polysaccharide composition, including the accumulation of ectopic callose. dgl1-1 did not exhibit a cellulose deficiency. the xyloglucan structure was not altered by the mutation of DGL1. |
| Impact on Sugar Composition | Despite the strong dwarf phenotype, the mutation did not affect the amount of synthesized cell wall polysaccharides. The sugar composition mainly showed an increase of arabinose and glucose in the non-cellulosic polymers in dgl1-1 compared with the wild type. |
| Reference | Lerouxel O, Mouille G, Andeme-Onzighi C, Bruyant MP, Seveno M, Loutelier-Bourhis C, Driouich A, Hofte H, Lerouge P. Mutants in DEFECTIVE GLYCOSYLATION, an Arabidopsis homolog of an oligosaccharyltransferase complex subunit, show protein underglycosylation and defects in cell differentiation and growth. Plant J. 2005 May;42(4):455-68. |