Mutant Name: eld1(elongation defective 1)
| Gene Name | KOB1 |
| Protein Name | elongation defective 1 protein |
| Mutagen | EMS, T-DNA |
| Category | forward genetics |
| Organism | Arabidospis thaliana |
| Ecotype | Col |
| Donor | |
| General Description |
1. ELD1 is the same gene as KOBITO1 and encodes a serine-rich protein conserved in higher plants. 2. eld1 mutants display stronger phenotype relative to the kob1 mutants and are likely null alleles. 3. eld1 mutants were dwarfed, had a very short root, and produced lateral organs, such as leaves, that were extremely reduced in size compared to wild type. 4. The eld1 mutants are seedling lethal in soil. 5. Dark-grown mutants had extremely short hypocotyls, underwent elaborate shoot development and displayed photomorphogenesis. |
| Organ Specificity | ELD1-GFP is localized to the cell wall/extracellular matrix, rather than the membrane, indicating that ELD1/KOB1 is a secreted protein involved in promoting cell growth. |
| Impact on Wall | Vascular differentiation also was severely impaired in eld1-2 as evidenced by the twisted vascular bundle and the presence of undifferentiated vascular cells. |
| Impact on Sugar Composition | White patches seen under a dissecting microscope, which were shown to be the result of ectopic suberin accumulation, present in root and hypocotyl of the eld1 mutants. |
| Reference | 1. Lertpiriyapong K, Sung ZR. The elongation defective1 mutant of Arabidopsis is impaired in the gene encoding a serine-rich secreted protein. Plant Mol Biol. 2003 Nov;53(4):581-95. |