Mutant Name: emb30(gnom)
| Gene Name | GN |
| Protein Name | SEC7 (homolog) |
| Mutagen | EMS,T-DNA |
| Category | forward genetics |
| Organism | Arabidopsis |
| Ecotype | Col |
| Donor | |
| General Description |
1.emb30-1 and emb30-2 mutagenized by EMS,ecotype columbia; emb30-2 and emb30-4 mutagenized by T-DNA insertion, ecotype wassilewskija. 2.EMB30 has been identified as a gene involved in embryogenesis and pattern formation. Later it was shown that mutations also affect the growth of undifferentiated plant cells and adult tissues. 3.Cell division, expansion, and adhesion are abnormal in emb30 leaves in comparison with wild-type leaves, when both were induced on SIM. 4.Mutant also showed cell wall defect and abnormal localization of pectin. |
| Organ Specificity | The EMB30 gene is expressed throughout the plant. |
| Impact on Wall |
1.emb30 mutants have a cell wall defect that sometimes allows material to be deposited into the interstitial space between cells instead of being restricted to cell corners. 2.In addition, pectin, a complex polysaccharide important for cell adhesion, appears to be abnormally localized in emb30 plants. In contrast, localization of epitopes associated with xyloglucan or arabinogalactan was similar in wild-type and emb30 tissues, and the localization of a marker molecule to vacuoles appeared normal. |
| Impact on Sugar Composition | |
| Reference |
1.Shevell DE, Kunkel T, Chua NH. Cell wall alterations in the arabidopsis emb30 mutant. Plant Cell. 2000 Nov;12(11):2047-60. 2. Shevell DE, Leu WM, Gillmor CS, Xia G, Feldmann KA, Chua NH. EMB30 is essential for normal cell division, cell expansion, and cell adhesion in Arabidopsis and encodes a protein that has similarity to Sec7. Cell. 1994 Jul 1;77(7):1051-62. |