Mutant Name: fdh(fiddlehead)
| Gene Name | FDH |
| Protein Name | FIDDLEHEAD |
| Mutagen | EMS, transposon, T-DNA |
| Category | forward genetics |
| Organism | Arabidopsis thaliana |
| Ecotype | Lb |
| Donor | |
| General Description |
1. In fdh-1 mutants, epidermal cells manifest not only a contact-mediated fusion response but also interact with pollen. 2.The FDH gene encodes a protein that is probably involved in the synthesis of long-chain lipids found in the cuticle and shows similarity to a large class of genes encoding proteins related to beta-ketoacyl-CoA synthases and chalcone synthases. |
| Organ Specificity | 1.FDH expression is limited to the aboveground parts of the plant. FDH mRNA is localized to epidermal cells. FDH mRNA is not detected in the septum of the ovary or in the stigmatic papillar cells. Interestingly, FDH mRNA can also be detected in phloem tissue. |
| Impact on Wall | fdh-1 tissues showed greater permeability to chemical fixatives than wild-type tissues and tended to be bleached more rapidly when immersed in alcohol. |
| Impact on Sugar Composition |
1. In contrast to wildtype plants, washes from fdh-1 leaf surfaces can be bound by AAA lectin, which detects alpha (1,6)-linked fucose residues in complex N-glycan structures. 2.The long chain fatty acids shared elevated abundances in fdh-derived walls compared to wild-type, while lower molecular weight lipids were underrepresented in fdh-derived walls. |
| Reference |
1.Lolle SJ, Berlyn GP, Engstrom EM, Krolikowski KA, Reiter WD, Pruitt RE. Developmental regulation of cell interactions in the Arabidopsis fiddlehead-1 mutant: a role for the epidermal cell wall and cuticle. Dev Biol. 1997 Sep 15;189(2):311-21. 2. Pruitt RE, Vielle-Calzada JP, Ploense SE, Grossniklaus U, Lolle SJ. FIDDLEHEAD, a gene required to suppress epidermal cell interactions in Arabidopsis, encodes a putative lipid biosynthetic enzyme. Proc Natl Acad Sci U S A. 2000 Feb 1;97(3):1311-6. |