Mutant Name: fra3
| Gene Name | FRA3 |
| Protein Name | Inositol Polyphosphate 5-Phosphatase-Type II |
| Mutagen | EMS |
| Category | forward genetics |
| Organism | Arabidopsis thaliana |
| Ecotype | Col |
| Donor | |
| General Description |
1.The fra3 mutant was isolated based on its reduced fiber strength phenotype. 2.Anatomical examination revealed that the fra3 mutation cause a dramatic reduction in secondary wall thickness, an alteration in actin organization, and an increase in PtdIns(4,5)P2 and Ins(1,4,5)P3 levels. 3.The FRA3 protein is composed of two domains, an N-terminal localized WD-repeat domain and a C-terminal localized 5PTase catalytic domain. 4.The FRA3 gene was mapped to BAC clone F5I14.11 gene |
| Organ Specificity | The FRA3 gene was found to be highly expressed in fiber cells and vascular tissues in stems. |
| Impact on Wall | 1.The fra3 mutations caused a dramatic reduction in secondary wall thickness and a concomitant decrease in stem strength. These phenotypes were associated with an alteration in actin organization in fiber cells. |
| Impact on Sugar Composition | |
| Reference | 1. Zhong R, Burk DH, Morrison WH 3rd, Ye ZH. FRAGILE FIBER3, an Arabidopsis gene encoding a type II inositol polyphosphate 5-phosphatase, is required for secondary wall synthesis and actin organization in fiber cells. Plant Cell. 2004 Dec;16(12):3242-59. |