Mutant Name: gsl5-1
| Gene Name | GSL05 |
| Protein Name | callose synthase |
| Mutagen | T-DNA |
| Category | forward genetics |
| Organism | Arabidopsis thaliana |
| Ecotype | Col |
| Donor | |
| General Description |
1.The phenotypic characteristics of the gsl5 lines(T-DNA insertion line GABI-KAT 089H05 ) were similar to those described for the powdery mildew resistant pmr4-1 mutants. 2.Although papillary callose was not detectable in gsl5 plants, the typical round wall appositions that form beneath fungal appressoria, at least at the light microscopic level, were indistinguishable from those in wild-type plants except that they contained no callose. |
| Organ Specificity | |
| Impact on Wall | |
| Impact on Sugar Composition | Despite the absence of callosic plugs in gsl5-1 mutant plants, round papillae still were clearly recognizable beneath fungal appressoria . the absence of heavy GSL5 callose deposition at cell margins in gsl5-1 plants reveals the underlying plasmodesmata callose. |
| Reference | Jacobs AK, Lipka V, Burton RA, Panstruga R, Strizhov N, Schulze-Lefert P, Fincher GB. An Arabidopsis Callose Synthase, GSL5, Is Required for Wound and Papillary Callose Formation. Plant Cell. 2003 Nov;15(11):2503-13. |