Mutant Name: irx2-1 (irregular xylem 2-1)
| Gene Name | IRX2 |
| Protein Name | endo-1,4-glucanase |
| Mutagen | EMS |
| Category | forward genetics |
| Organism | Arabidopsis thaliana |
| Ecotype | Lb |
| Donor | |
| General Description |
1.irx2-1 is an allele of korrigan. Pro-250 is altered to Leu. 2.It has a cellulose deficiency in the secondary cell wall with a decrease in stiffness of the stem material. |
| Organ Specificity |
1.The inflorescence stem of irx2 mutant contains approximately 30% of the total crystalline cellulose of the wild type. 2. No decrease in cellulose was observed in the primary walls of cortical and epidermal cells of the stem. |
| Impact on Wall | It has a role in the later stages of secondary cell wall formation, maybe in processing of the growing microfibrils or release of the cellulose synthase complex. |
| Impact on Sugar Composition | The total crystalline cellulose in the inflorescence stem of irx2 mutant contains approximately 30% of the level of the wild type. |
| Reference |
1.Szyjanowicz PM, McKinnon I, Taylor NG, Gardiner J, Jarvis MC, Turner SR. (2004) The irregular xylem 2 mutant is an allele of korrigan that affects the secondary cell wall of Arabidopsis thaliana. Plant J. Mar;37(5):730-40. 2.Turner, S.R. and Somerville, C.R. (1997) Collapsed xylem phenotype of Arabidopsis identifies mutants deficient in cellulose deposition in the secondary cell wall. Plant Cell, 9, 689-701. 3.Nicol F, His I, Jauneau A, Vernhettes S, Canut H, Hofte H. (1998) A plasma membrane-bound putative endo-1,4-beta-D-glucanase is required for normal wall assembly and cell elongation in Arabidopsis. EMBO J. Oct 1;17(19):5563-76. |