Mutant Name: ixr1
| Gene Name | CesA3 |
| Protein Name | Cellulose synthase |
| Mutagen | EMS |
| Category | forward genetics |
| Organism | Arabidopsis thaliana |
| Ecotype | Col |
| Donor | |
| General Description |
1. The mutant has resistance against herbicide isoxaben; 2. It is semi-dominant; 3. The two mutant alleles contain point mutations that replace glycine 998 with aspartic acid, and threonine 942 with isoleucine, respectively. The mutations occur in a highly conserved region of the enzyme near the carboxyl terminus that is well separated from the proposed active site. |
| Organ Specificity |
1. Northern blots showed IXR1 was highly expressed in stems, flowers, roots, and shoots. 2. In transgenic plants containing the RSW1:GUS promoter fusion, the root cap and the epidermal layer had high levels of GUS activity, but activity was not detected in the internal cells of the meristem region of the root. All of the cells of the root above the meristematic region exhibited strong GUS activity. |
| Impact on Wall | |
| Impact on Sugar Composition | |
| Reference |
1.Heim D.R., Roberts J.L., Pike P.D., Larrinua I.M., (1989)Mutation of a locus of Arabidopsis thaliana confers resistance to the herbicide isoxaben. Plant Physiology 90 (1): 146-150. 2. Scheible WR, Eshed R, Richmond T, Delmer D, Somerville C. (2001) Modifications of cellulose synthase confer resistance to isoxaben and thiazolidinone herbicides in Arabidopsis Ixr1 mutants. Proc Natl Acad Sci U S A. Aug 28;98(18):10079-84. |