Mutant Name: knopf (knf)
| Gene Name | KNF |
| Protein Name | alpha-Glucosidase I |
| Mutagen | EMS |
| Category | forward genetics |
| Organism | Arabidopsis thaliana |
| Ecotype | Lb |
| Donor | |
| General Description | 1. KNOPF is required for the orientation of cell elongation during embryogenesis. 2. knf mutant seedlings are not able to elongate or grow to any extent. 3. Loss of the splice donor site in knf-14 introduces an in-frame stop codon. 4. result in a loss of trimming of N-linked glycans. 5. KNOPF is not directly involved in cellulose synthesis. |
| Organ Specificity | 1. radially swollen during embryogenesis with very abnormal width of the cells. |
| Impact on Wall | 1. has thin epidermal cell walls. 2. The outer part of the cell junction is disorganized and lumpy, and the thickness of the wall changes abruptly. |
| Impact on Sugar Composition | 1. cell walls are deficient in cellulose: 13% of wt in embryos. 2. levels of fucose is decreased to 60% of wt. |
| Reference | 1. C. Stewart Gillmor, Patricia Poindexter, Justin Lorieau, Monica M. Palcic, and Chris Somerville. Alpha-Glucosidase I is required for cellulose biosynthesis and morphogenesis in Arabidopsis. J. Cell Biol. 2002 156: 1003-1013. |