Mutant Name: mur3
| Gene Name | MUR3 |
| Protein Name | Xyloglucan-galactosyl transferase |
| Mutagen | EMS |
| Category | forward genetics |
| Organism | Arabidopsis thaliana |
| Ecotype | Col |
| Donor | |
| General Description |
1.L-fucose content is reduced to 50%of the wild type. 2.Plants were phenotypically normal and exhibited tensile strength in their inflorescence stems comparable to that of wild-type plants. |
| Organ Specificity | L-fucose content in aerial organs reduced to 50% of the wild type. The change in XyG structure affected all major plant organs. |
| Impact on Wall | mur3 mutation affects the monosaccharide composition of XyG. Plant exhibited tensile strength in their inflorescence stems |
| Impact on Sugar Composition |
1.Mutants have 50% reduction in cell wall fucose content. 2.mur3 defect results in a failure of attachment of the Gal residue on the third xylosyl unit within the XXXG core structure. This failure severely alters the XyG structure in two ways. First, the L-Fuc-(1,2)-D-Gal- side group, which is considered important for XyG binding to cellulose, is completely absent. Second, galactosylation at the second xylose residue is enhanced. |
| Reference |
1. Reiter WD, Chapple CCS, Somerville CR (1997) Mutant of Arabidopsis thaliana with altered cell wall polysaccharide composition. Plant J 12: 335-345 2. Madson M, Dunand C, Li X, Verma R, Vanzin GF, Caplan J, Shoue DA, Carpita NC, Reiter WD.(2003) The MUR3 gene of Arabidopsis encodes a xyloglucan galactosyltransferase that is evolutionarily related to animal exostosins. Plant Cell. Jul;15(7):1662-70. |