Mutant Name: nolac-H14
| Gene Name | GUT1 |
| Protein Name | glucoronyltransferase |
| Mutagen | T-DNA |
| Category | forward genetics |
| Organism | Nicotiana plumbaginifolia |
| Ecotype | Others |
| Donor | |
| General Description | The cells of nolac-H14(nonorganogenic callus with loosely attached cells) callus were somewhat larger than those of normal organogenic callus and were loosely attached, and the morphology of the cell clusters was irregular. |
| Organ Specificity | |
| Impact on Wall |
1.Transmission electron microscopy revealed that the middle lamellae and the cell walls of nolac-H14 were barely stained by ruthenium red, even after demethylesterification with NaOH, whereas the entire cell wall and the middle lamella were strongly stained in normal callus. 2.When compared with normal callus, in nolac-H14 callus cell walls contained reduced amounts of pectin, especially in hemicellulosic fractions, and exhibited increased amounts of extracellular polysaccharides. |
| Impact on Sugar Composition |
1.The amounts of arabinose and galacturonic acid in nolac-H14 callus were 40% and 50%, respectively, of the amounts in normal callus. 2.The ratio of arabinose to galactose was low in the pectic polysaccharides purified from all cell wall fractions and from the medium, in particular, in the hemicellulose fractions. |
| Reference | Iwai H, Ishii T, Satoh S. Absence of arabinan in the side chains of the pectic polysaccharides strongly associated with cell walls of Nicotiana plumbaginifolia non-organogenic callus with loosely attached constituent cells. Planta. 2001 Oct;213(6):907-15. |