Mutant Name: nolac-H18
| Gene Name | GUT1 |
| Protein Name | glucuronyltransferase 1 |
| Mutagen | T-DNA |
| Category | forward genetics |
| Organism | Nicotiana plumbaginifolia |
| Ecotype | Others |
| Donor | |
| General Description |
1.The cells of nolac-H18(nonorganogenic callus with loosely attached cells) callus were somewhat larger than those of normal organogenic callus and were loosely attached, and the morphology of the cell clusters was irregular. 2.The nolac-H18 mutant contains a T-DNA insertion in exon 1 of the NpGUT1 gene between amino acids Y89 and Q90. |
| Organ Specificity | NpGUT1 mRNA was abundant in apical shoots and roots, but not in leaves. |
| Impact on Wall | Clusters of nolac-H18 cells were disorganized with random morphology and weak intercellular attachment. |
| Impact on Sugar Composition | The glucuronic acid (GlcA) content in nolac-H18 callus was drastically reduced to 14% of that of normal callus, whereas the levels of the other sugars were reduced by as much as 60%. |
| Reference | Iwai H, Masaoka N, Ishii T, Satoh S. A pectin glucuronyltransferase gene is essential for intercellular attachment in the plant meristem. Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16319-24. Epub 2002 Nov 25. |