Mutant Name: pen(penetration)1
| Gene Name | PEN1 |
| Protein Name | Syntaxin, SYP121 |
| Mutagen | EMS |
| Category | forward genetics |
| Organism | Arabidopsis thaliana |
| Ecotype | Col |
| Donor | |
| General Description |
1.PEN1 is required for basal penetration resistance and non-host resistance against barley powdery mildew. 2.The onset of papillae formation was found to be significantly delayed in the pen1-1 mutant. 3.The pen1/syp122-1 double mutant is both severely dwarfed and necrotic. |
| Organ Specificity |
1.Expression of PEN1's closest homologue, SYP122, is responsive to infection. SYP122 is significantly expressed in vegetative tissues. 2.GFP-PEN1 and CFP-SYP122 fusion proteins were shown to be both intact and functional and localized to the plasma membrane. Upon infection with both the host and nonhost powdery mildews, GFP-PEN1 and CFP-SYP122 accumulate at papillae. |
| Impact on Wall | 1.The syp122-1 mutant has a primary cell wall defect. |
| Impact on Sugar Composition | 1.The pen1 / syp122-1 double mutants, harvested before or after lesioning, showed a complex change in cell wall monosaccharide content, with a slight but significant (p < 0.03) increase in fucose, arabinose, and mannose. |
| Reference |
1. Collins, N.C. et al. (2003). SNARE-protein-mediated disease resistance at the plant cell wall. Nature 425, 973-977. 2. Assaad, FF et al. The PEN1 Syntaxin Defines a Novel Cellular Compartment upon Fungal Attack and Is Required for the Timely Assembly of Papilla. Mol Biol Cell. Vol. 15, Issue 11, 5118-5129, November 2004 |