Mutant Name: pmr6
| Gene Name | PMR6 |
| Protein Name | pectate lyase-like protein |
| Mutagen | EMS, T-DNA |
| Category | forward genetics |
| Organism | Arabidopsis thaliana |
| Ecotype | Col |
| Donor | |
| General Description |
1. The pmr6 mutant is resistant to powdery mildew, requiring neither salicylic acid nor the ability to perceive jasmonic acid or ethylene. 2. PMR6 contains a pectate lyase-like domain fused to a novel 84-amino acid C-terminal domain. |
| Organ Specificity |
1. PMR6 mRNA is expressed approximately equally in all organs tested: mature leaves, flowers/buds, rosettes, and roots. PMR6 mRNA levels were not affected significantly by powdery mildew infection at 3 DAI. 2. pmr6 leaves were shorter, rounder, and cupped slightly upward compared with wild-type leaves. |
| Impact on Wall | Cell walls of mutant plants are enriched for pectins with a lower degree of esterification and an alteration in the H bonding environment of cellulose microfibrils. |
| Impact on Sugar Composition |
1. pmr6-1 cell walls are enriched for pectins with a lower degree of esterification and an alteration in the H bonding environment of cellulose microfibrils. 2. pmr6-1 cellulose forms a different hydrogen bond network than does wild-type cellulose or that the rotational environment of the -CH2OH group of cellulose has changed. |
| Reference | 1. Vogel JP, Raab TK, Schiff C, Somerville SC. PMR6, a pectate lyase-like gene required for powdery mildew susceptibility in Arabidopsis. Plant Cell. 2002 Sep;14(9):2095-106. |