Mutant Name: prc1-1(procuste1)
| Gene Name | CesA6 |
| Protein Name | cellulose synthase |
| Mutagen | EMS |
| Category | forward genetics |
| Organism | Arabidopsis thaliana |
| Ecotype | Col |
| Donor | |
| General Description |
1.Mutants showed decreased cell elongation, specifically in roots and dark-grown hypocotyls. 2.Cell elongation defects are correlated with a cellulose deficiency and the presence of gapped walls. 3.Prc1 mutants carried a point mutation or a deletion in the CesA6 gene carried by P1 clone MVP7. |
| Organ Specificity |
1.PRC1 mRNA were detected in all organs investigated, except in mature flowers. 2.In developing seedlings, PRC1 mRNAs were high initially and decreased at later growth stages. Expression was high in elongating stems but dropped in the stem sections in which secondary wall synthesis takes place. |
| Impact on Wall |
1.Mutants hypocotyls were larger than wild-type hypocotyls, with more radially expanded cells in epidermis, cortex, and endodermis. 2.Incomplete walls were found in epidermal, cortical, and endodermal cell layers. |
| Impact on Sugar Composition |
1.chemical analysis of the cell walls of mutant seedlings confirms the deficiency in cellulose observed by FTIR microspectroscopy. 2.Other changes detected included a relative increase in hemicellulosic polysaccharides and qualitative changes in pectin composition. 3. a slight accumulation of callose in DCB-treated seedlings was also observed. |
| Reference |
1. Fagard M, Desnos T, Desprez T, Goubet F, Refregier G, Mouille G, McCann M, Rayon C, Vernhettes S, Hofte H. PROCUSTE1 encodes a cellulose synthase required for normal cell elongation specifically in roots and dark-grown hypocotyls of Arabidopsis.Plant Cell. 2000 Dec;12(12):2409-2424. 2.Desnos T, Orbovic V, Bellini C, Kronenberger J, Caboche M, Traas J, Hofte H., Procuste1 mutants identify two distinct genetic pathways controlling hypocotyl cell elongation, respectively in dark- and light-grown Arabidopsis seedlings. Development. 1996 Feb;122(2):683-93. |