Mutant Name: rat1
| Gene Name | AGP17 |
| Protein Name | Arabinogalactan-protein17 |
| Mutagen | T-DNA |
| Category | forward genetics |
| Organism | Arabidopsis thaliana |
| Ecotype | Ws |
| Donor | |
| General Description |
1. The mutant is resistant to Agrobacterium transformation. 2. rat1 phenotype results from a defect in the loose or initial binding step in Agrobacterium infection. 3. T-DNA tag is 1,097 bp upstream of the ATG start codon of AtAGP17. |
| Organ Specificity |
1. RAT1 is expressed in flower tissue and root tissue. But the expression in root is very low. 2. The rat phenotype of rat1 is caused by reduced AGP17 expression in roots. |
| Impact on Wall | Agrobacterium binding is reduced, with only occasional binding on the root surface and at the root hair tips. |
| Impact on Sugar Composition | |
| Reference |
1. Yolanda Maria Gaspar, Jaesung Nam, Carolyn Jane Schultz, Lan-Ying Lee, Paul R. Gilson, Stanton B. Gelvin, and Antony Bacic. (2004) Characterization of the Arabidopsis Lysine-Rich Arabinogalactan-Protein AtAGP17 Mutant (rat1) That Results in a Decreased Efficiency of Agrobacterium Transformation. Plant Physiol. 135: 2162-2171. 2.Zhu Y, Nam J, Humara J, Mysore K, Lee L-Y, Cao H, Valentine L, Li J, Kaiser A, Kopecky A, et al (2003a) Identification of Arabidopsis rat mutants. Plant Physiol 132: 494?505. 3.Nam J, Mysore KS, Zheng C, Knue MK, Matthysse AG, Gelvin SB (1999) Identification of T-DNA tagged Arabidopsis mutants that are resistant to transformation by Agrobacterium. Mol Gen Genet 261: 429?438. |