Mutant Name: rat4
| Gene Name | CSLA9 |
| Protein Name | mannan synthase |
| Mutagen | T-DNA |
| Category | forward genetics |
| Organism | Arabidopsis thaliana |
| Ecotype | Ws |
| Donor | |
| General Description |
1. The mutant is resistant to Agrobacterium transformation. 2. It contains a T-DNA insertion into the 3'-untranslated region of CSLA9 with a greatly reduced transcription level. 3. It has reduced numbers and rate of growth of lateral roots and reduced ability of the roots to bind A. tumefaciens cells under certain conditions. |
| Organ Specificity |
1. In young plants (27 d old), GUS expression driven by RAT4 promoter was observed primarily in the cotyledons, at the base of the hypocotyls, in newly emerging lateral root primordia, and in the root elongation zone. 2. As the plant matured, GUS expression driven by RAT4 promoter in the aerial portions of the plant was primarily in the vascular system of young leaves, in the abscission zone of the pedicle and in the flower receptacle. 3. When grown in vitro on agar, 2 weeks old rat4 plants developed approximately one-half the number of lateral roots as did wild-type plants with a visible difference in length. |
| Impact on Wall | 1. The has a general surface defect that affects binding of diverse bacteria. |
| Impact on Sugar Composition | The mutant has no major alterations in cell wall linkage composition and only a small increase in Gal and Gal-linked polymers. |
| Reference | 1. Zhu Y, Nam J, Carpita NC, Matthysse AG, Gelvin SB. (2003) Agrobacterium-mediated root transformation is inhibited by mutation of an Arabidopsis cellulose synthase-like gene. Plant Physiol. Nov;133(3):1000-10. |