Mutant Name: rsw1-2
| Gene Name | CesA1 |
| Protein Name | Cellulose synthase |
| Mutagen | EMS |
| Category | forward genetics |
| Organism | Arabidopsis thaliana |
| Ecotype | Lb |
| Donor | |
| General Description |
1. rsw1-2 is a nonconditional mutant allele of the CesA1 gene. 2.rsw1-2 is a stronger mutant allele than rsw1-1. 3. rsw1-2 has a single G to A change at bp 1891 of the coding sequence and results in a glycine to serine change at amino acid residue 631 of the CESA1 protein, a residue that is conserved and near the proposed catalytic site. 4. RSW1 is required for the orientation of cell elongation during embryogenesis. |
| Organ Specificity |
1.rsw1-2 displays a more severe phenotype than rsw1-1; the phenotype is apparent in all tissues of the seedling at all growth temperatures. Cotyledons are turgid, elongated petioles are not apparent, and the hypocotyl and roots are a swollen mass. 2. radially swollen during embryogenesis with abnormal width of the cells. |
| Impact on Wall | Mutants have thin epidermal cell walls. |
| Impact on Sugar Composition | cell walls are deficient in cellulose: 22% of wt in embryos. |
| Reference | C. Stewart Gillmor, Patricia Poindexter, Justin Lorieau, Monica M. Palcic, and Chris Somerville. Alpha-Glucosidase I is required for cellulose biosynthesis and morphogenesis in Arabidopsis. J. Cell Biol. 2002 156: 1003-1013. |