Mutant Name: rsw3
| Gene Name | RSW3 |
| Protein Name | glucosidase II |
| Mutagen | EMS |
| Category | forward genetics |
| Organism | Arabidopsis thaliana |
| Ecotype | Col |
| Donor | |
| General Description |
rsw3 is a temperature-sensitive mutant showing radially swollen root and a deficiency in cellulose. The mutation is present in a gene encoding a putative alpha-subunit of glucosidase II. 2. This glucosidase functions in processing N-linked glycans in the ER. |
| Organ Specificity | root |
| Impact on Wall |
1.The cells in rsw3 were only slightly shorter than wild type1. Cell division seems more strongly affected than cell expansion. 2. At non-permissive temperature, the mutant has reduced cellulose synthesis in primary walls. Most features of the rsw3 phenotype are shared with other cellulose deficient mutants, but some are not, e.g. lack of secreted seed mucilage. The lack of glucosidase II likely affects processes other than cellulose synthesis. |
| Impact on Sugar Composition | reduced cellulose synthesis . |
| Reference |
1.Joanne E. Burn, Ursula A. Hurley, Rosemary J. Birch, Tony Arioli , Ann Cork and Richard E. Williamson. The cellulose-deficient Arabidopsis mutant rsw3 is defective in a gene encoding a putative glucosidase II, an enzyme processing N-glycans during ER quality control.The Plant Journal.2002,32(6):949 2.Peng, L., Hocart, C.H., Redmond, J.W., and Williamson, R.E. (2000). Fractionation of carbohydrates in Arabidopsis root cell walls shows that three radial swelling loci are specifically involved in cellulose production. Planta 211, 406-414. |